Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase
نویسندگان
چکیده
منابع مشابه
Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase.
Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activ...
متن کاملAssociation of inorganic-pyrophosphatase activity with human alkaline-phosphatase preparations.
1. The inorganic-pyrophosphatase activity of alkaline phosphatases prepared from human liver and small intestine was investigated at different stages of purification. 2. Both liver and intestinal preparations possessed pyrophosphatase activity at all stages of purification, and the two types of activity were not separated by gel filtration or by anion-exchange or cation-exchange chromatography....
متن کاملLocalization of Alkaline Phosphatase in Mammalian Bones
ALTHOUGH it is well established that alkaline phosphatase is present . Z J L in high concentrations in mammalian bones, the histological and cytological distribution of the enzyme in normal adult bones has not been adequately studied. Since the introduction of techniques for the histochemical localization of phosphatase (Gomori, 1939; Takamatsu, 1939) a number of papers have been published on t...
متن کاملAlkaline Phosphatase Activity of Leukocytes in Shock
By FRANCESCO VACCARI, BENITO SABOTTO AND ENRICO MANZINI T HE ROLE of the alkaline phosphatases in organic metabolism is riot yet clear. In addition to their role in nucleoprotein metabolism and in the process of ossification, they are also involved in glycogemi synthesis from hmexose diphosphate’ and presumably in gluconeogenesis from proteins.2 Their widespread distribution in the tissues, how...
متن کامل[Fine structural demonstration of alkaline phosphatase activity].
For the application of Glenner's tetrazolium method to the electron histochemical demonstration of monoamine oxidase some in vitro examination were carried out with special referance to indentification and efflux of formazan and intracellular localization of the enzyme in the locus coeruleus of the rat brain was studied. The results of examinations in vitro are summerized as follows: 1. Formaza...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0306-3283
DOI: 10.1042/bj1050155